![]() ![]() ab32150 1:500), anti-rabbit monoclonal procaspase-6 (cat. ab32503 1:1,000),Īnti-rabbit monoclonal B cell lymphoma-extra large (Bcl-xL cat. Anti-rabbit monoclonal B cell lymphoma-2Īssociated X, apoptosis regulator (Bax cat. ![]() Medium, fetal bovine serum (FBS) and penicillin/streptomycinĪntibiotics were obtained from Gibco Thermo Fisher Scientific, MTT reagent and dimethyl sulfoxide (DMSO) were ordered from SigmaĪldrich Merck KGaA (Darmstadt, Germany). Materials and methods Materials and reagents Of bakuchiol in gastric cancer, aiming to produce novel insights toĮnhance understanding of the underlying molecular mechanism. Multiple biological uses including the treatment of cancer and HIVĭemonstrated to exhibit cytotoxicity in certain human cancer cellĮxaminations, the present study investigated the anticancer effects Previous reports have demonstrated that molecular structuresĬontaining styryl moieties in conjugation with other structuralįeatures, including chromones, quinazolines and pyrenes, have Psoralea corylifolia, a member of the Leguminosae family. Typical prenylated monoterpene phenolic compound separated from Novel, effective therapeutic compounds continues. Source of medicinal agents useful to treat humans, and the hunt for Plant-derived natural products have been used as a Therefore, there is a necessity to establish novel therapeuticĪgents that diminish the mortality rate of patients with gastric Remains a distinctive lack of targeted treatments in the clinical Mechanisms underlying gastric cancer have improved, but there Mortality and considered as the foremost gastrointestinal Still ranked as the second major cause of cancer-associated Prevalence and mortality over the past 20 years, gastric cancer is (10–12%) incidence rate in Asia and Europe ( 1). It is estimated that gastric cancer alone causesĪlmost 10% of all cancer-associated mortality with a higher Gastric cancer is one of the most common malignantĬancers globally. These findings demonstrated that bakuchiol possesses potential for treating human gastric cancer. Bakuchiol‑induced cell death was mitochondrial dependent, through modulation of phosphoinositide 3‑kinase/AKT and mitogen‑activated protein kinase signaling pathways. Bakuchiol‑treated NUGC3 cells demonstrated significantly reduced phosphorylated (p‑) protein kinase B (AKT) protein expression levels and elevated p‑extracellular signal related kinase 1/2 (ERK1/2), p‑c‑Jun N‑terminal kinase (JNK) and p‑p38. Western blotting results indicated that bakuchiol treatment significantly decreased procaspase‑3,6,8,9 and poly (ADP‑ribose) polymerase (PARP) expression levels, increased cleaved caspase‑3 and cleaved PARP expression levels, and increased the B cell lymphoma‑2 associated X, apoptosis regulator:B cell lymphoma‑extra large ratio. Nuclear fragmentation and the formation of apoptotic organelles were promoted in bakuchiol‑treated NUGC3 cells. In addition, bakuchiol significantly increased the apoptotic cell population in the sub‑G1 phase, and Annexin‑V‑fluorescein isothiocyanate/propidium iodide double staining confirmed the increase in apoptosis. Bakuchiol treatment significantly inhibited NUGC3 human gastric cancer cell viability in a concentration dependent manner. The primary aim of the present study was to identify the molecular mechanisms underlying the anticancer effect of bakuchiol monoterpenes. ![]() Bakuchiol is extracted from Psoralea corylifolia, a member of the Leguminosae family, has been used in Indian Ayurvedic and Chinese traditional medicine, and it possesses an anticancer effect. ![]()
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